Selection of tumor cell lines in nude mice
The selection of tumor cell lines is indeed a very troublesome problem. There are many cell lines mentioned in various literatures, but in general, I think that for our nude mouse tumor model, the selection of cell lines should be considered The following aspects:
1. International recognition. Although SFDA's guidelines indicate that any cell line can be established, generally speaking, more internationally recognized cell lines should be considered as much as possible.
2. The growth situation in vivo, many cell lines have a high appearance rate in the literature, but in fact, they are basically known from the outside of the body, so many people put on the body hard, and the result is that it is not easy, such as The A549 mentioned by the landlord, as well as MCF-7, are all well-known cell lines, but they are not a good test object in vivo. The tumor formation rate is low, the growth is slow, and it is evenly poor. At present, many domestic R & D units, especially the leaders of some companies, lack of knowledge in this regard, hang these famous cell lines at their lips, and firmly press the front-line researchers to work hard to make these cell lines. It ’s not good, so I doubt everyone ’s level. Grandma ’s, I ’m very angry about it!
3. The sensitivity of the drug is often overlooked by everyone. Most nude mouse tumor models are for the screening and research of anti-tumor drugs, but often everyone only considers whether this cell line is good, but there is no Considering the sensitivity of the cell line itself to drugs, such as Lovo cells, international recognition is also okay, and the growth in vivo is also great, but the sensitivity to drugs is not good, most of the conventional chemotherapy drugs come to it, tumor suppression The rate will decline, obviously, it is not a good choice for the screening of anti-tumor drugs.
In addition, the relationship between the body and the body. Now people in the body are often led by people outside the body. What cell strains are made outside the body? People in the body have to do this, but they are alive and dead. It ’s not good. Actually, the point of view between me and some seniors is that the selection of cell lines should be coordinated in vivo and in vitro. There are a lot of cell lines in the hands of people in vitro. If the people in the body do not go They coordinate, so they will always be led by the nose, and it ’s not pleasing to get involved. You should sit down and choose 20 to 30 cell lines together as a routine screening cell line. This is enough. Consider the direction when selecting cell lines. (Stomach cancer, liver cancer, lung cancer or something), targets (EGF, vEGF, etc.),
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