Enzyme Linked Immunoassay (ELISA) Instructions for Mouse Estradiol (E2)

This reagent is for research purposes only: this kit is used to determine the content of estradiol (E2) in mouse serum, plasma, tissues and related liquid samples. Experimental principle: This kit uses the double antibody sandwich method to determine the level of estradiol (E2) in mice in the specimen. The microplate was coated with purified estradiol (E2) antibody to make a solid phase antibody. Estradiol (E2) was added to the monoclonal antibody-coated microwells and then combined with HRP-labeled goat anti-mouse antibody The antibody-antigen-enzyme-labeled antibody complex is thoroughly washed and added with substrate TMB for color development. TMB is converted into blue under the catalysis of HRP enzyme, and into the final yellow under the action of acid. The color depth is positively correlated with the estradiol (E2) in the sample. The absorbance (OD value) was measured with a microplate reader at a wavelength of 450 nm, and the concentration of mouse estradiol (E2) in the sample was calculated by a standard curve.

Dog House

sample is available for plush dog house,plush pet house,wire pet house and etc.

welcome to discuss the details,we will give the professional suggestion and sent the sample for check the quality.

Dongyang Yadi Pet Product Co , Ltd was founded in 2006 and covers an area of 6500 square meters . We are located in Zhejiang , the famous manufacturing city in China.We are specialized in developing and manufacturing many kinds of pet products. Our featured products are pet collar ,PET Leash ,pet harness, pet clothes,pet bandana,small pet products,ect.

Our company also o ffer all kinds of OEM/ODM services.And enjoy a good reputation among clients,we can provide you with the highest quality products and services.

In order to take up the market , our company focuses on introducing and cultivating talents as well as constantly developing new products.We always take the principle of [credibility , concentrate on work , quality supreme , innovation and development", with a heart of thanks giving to return customers and society.

Welcome to visit our company!

Dog House,Plush Dog House,Plush Pet House,Wire Pet House

Dongyang Yadi Pet Product Co.,Ltd , http://www.dyyadipet.com