The neutrophils are the body's first-line barrier against damage or infection. Researchers have long believed that neutrophils have only a single function, which is to release antibacterial proteins and take up pathogens. Recent studies have confirmed that their functions are very complex and play a key role in the activation of chronic and acute inflammation, especially the immune system corresponding to injury. Studying the methods of gene expression and protein synthesis of neutrophils helps to understand the information about the immune response, but it has been quite difficult to collect neutrophils for analysis. The standard separation operation takes more than two hours and requires a large number of blood samples. The neutrophils are very sensitive to operation and can be easily activated, thus changing the expression of the target molecule. In addition, neutrophils contain only a very small amount of mRNA, which cannot meet the needs of gene expression research.
"Although many researchers and clinicians have great interest in neutrophils, it is not easy to collect and process central granulocytes." The first author of the paper, the Medical Engineering Center of Massachusetts General Hospital Dr. Kenneth Kotz said: "This tool allows us to conduct extensive research and diagnosis based on cell-specific genome and proteome markers." A research team at Massachusetts General Hospital (MGH) has developed a new microfluidic tool that can be The neutrophils are quickly and accurately separated from the blood sample, which provides the necessary information for a better understanding of the response of the human immune system to the injury. "Nature Medicine" magazine reported online this new cell separation tool.
Kotz's research team developed a new system based on the silicon chip used to separate CD4 T cells and circulating tumor cells from a centiliter of microliters in 5 minutes. Successfully collected neutrophil samples revealed changes in gene and protein activity related to cell activity.
Dr. Ronald Tompkins, director of the burn department at the University of Massachusetts, co-author of the paper, and initiator and principal of the "Inflammation and Injury Host Response" project, said: "This technology is widely used in our" cooperative research program " With a profound impact, it can quickly and accurately obtain specific cells in a routine clinical environment, which may change the normal clinical diagnosis mode. This natural medical research has received funding from the National Institutes of Health, 7 clinical clinics and 17 colleges Institutions are widely involved in research collaboration.
Although the laboratory test results are encouraging, samples of injured patients still need to be processed in a true clinical environment. Through the efforts of co-author Dr. Lyle Moldawer of the University of Florida School of Medicine, the device was applied to a national study that impaired immune response. The researchers used the device to analyze the blood samples of 26 patients with severe burns or other injuries at six different locations, revealing the complex transformation of gene expression in the 28 days after the injury, reflecting various immune systems. Complex interactions between components.
Kotz said: "Until now, there is no other research institution that can carry out research on neutrophils like us. This technology will soon be no longer limited to neutrophil separation, but can also be used to capture all Cell type, we are working to apply it to other cell analyses. "
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