Instruction Manual of Quantitative Detection Kit (ELISA) for Rat Hydroxyproline (Hyp)

Instruction Manual of Quantitative Detection Kit (ELISA) for Rat Hydroxyproline (Hyp)

【Rat Hydroxyproline (Hyp) Elisa Kit Kit Name】

Rat Hydroxyproline (Hyp) Quantitative Detection Kit (ELISA)

【Use of rat hydroxyproline (Hyp) Elisa kit kit】

Quantitatively detect the content of hydroxyproline (Hyp) in rat serum, plasma and related liquid samples.

[Test principle of rat hydroxyproline (Hyp) Elisa kit]

This kit uses double-antibody two-step sandwich enzyme-linked immunosorbent assay (ELISA). Add the standard and the test sample to the pre-coated rat hydroxyproline (Hyp) monoclonal antibody transparent enzyme label coated plate. After incubation for a sufficient time, wash to remove unbound components, and then add the enzyme label to work After incubation for a sufficient period of time, wash to remove unbound components. Substrates A and B were added in sequence, and the substrate (TMB) was converted into a blue product catalyzed by horseradish peroxidase (HRP), which turned yellow under the action of an acid. The concentration of Hyp is positively correlated. The OD value is measured at a wavelength of 450 nm. Based on the OD value of the standard and the sample, the content of Hyp in the sample is calculated.

[Composition of the rat hydroxyproline (Hyp) Elisa kit kit]

1

Enzyme coated plate

12 holes × 8

7

Developer A liquid

6mL

2

Standard

0.3mL × 6 tubes

8

Developer B liquid

6mL

3

20 times concentrated washing liquid

25mL

9

Stop solution

6mL

4

Sample diluent

6mL

10

Instructions

1 serving

5

Special diluent

6mL

11

Sealing film

2 sheets

6

Enzyme reagent

6mL

12

sealed bag

1

Remarks: The concentration of standard products (No. 1 → No. 6) is: 1200, 600, 300, 150, 75, 37.5 μg / L

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