Human interferon (IFN-γ) enzyme-linked immunoassay (ELISA)
Kit Instruction Manual This kit is for research use only.
Generic name: Human IFN-γ (IFN-γ) ELISA kit purpose:
This kit is used to determine the content of interferon-γ (IFN-γ) in human serum, plasma, or other related tissue fluids.
Experimental principle:
This kit uses the double antibody sandwich method to determine the level of human interferon gamma (IFN-γ) in the specimen. Using purified human interferon gamma
FN- γ) antibody coated microplate to make solid phase antibody, add interferon γ (IFN- γ) to the monoclonal antibody-coated microwells in turn, and then combine with HRP-labeled goat anti-human receptor to form antibody -Antigen-enzyme-labeled antibody complex, add substrate after thorough washing
MB color development. TMB is converted into blue under the catalysis of HRP enzyme, and into the final yellow under the action of acid. The color depth is positively correlated with interferon-gamma (IFN-γ) in the sample. Measure absorbance (OD) at 450 nm with a microplate reader
Value), calculate the content of human interferon-γ (IFN-γ) in the sample through the standard curve.
Shanghai Yueyan Biological Technology Co., Ltd. is one of the main suppliers of immunology products in China. It provides various original and sub-packaged detection ELISA kits with high quality and low price, and complete after-sales service. Any problem with the kit has technology The teacher will help you out. It can be tested on behalf of you for free, so as to serve you better. If you have any other questions, please contact us in time.
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