The nucleic acid protein detector is the main device for chromatographic analysis. The nucleic acid protein detector is equipped with a chromatography column, a constant current pump, a partial collector, a chromatographic analysis system (optional as needed) and a computer printing device to form a complete set. The nucleic acid protein detector separates the chromatography system. It is a modern analytical laboratory instrument for life science research, pharmaceutical measurement, chemical, food science and medical research. The nucleic acid protein detector analysis system is widely used in scientific research and teaching experiments in industry, agriculture, scientific research and universities.
The principle of the nucleic acid protein detector is that according to the characteristic that the substance (sample) has obvious absorption of ultraviolet light, the ratio analysis of the sample component content is realized, so as to carry out the identification detection and content determination of the sample protein and the nucleic acid substance. It provides a unique analytical tool for nucleic acid, protein detection, purification and extraction in biochemical analysis, environmental science, food research, toxicology research, and new drug development.
Ultraviolet analyzer
The principle of UV detection is based on the difference in absorbance between the measured component and the background electrolyte. When the detected component passes through the detection window, the change in absorbance is subject to Lambert-Beer law, that is, under certain experimental conditions, the absorbance and the measured component are The concentration is proportional.
UV analyzer for nucleic acid protein detection
Ultraviolet-visible spectrophotometers can be used in biotechnology to determine the concentration of nucleic acids and proteins.
First, the basic structural unit of a nucleic acid is a nucleotide. A nucleotide consists of a nitrogenous base (purine or pyrimidine), a pentose (ribose or deoxyribose) and one or several phosphates. Since the base of the nucleic acid has a conjugated double bond, it has a property of ultraviolet absorption. The absorption spectra of various bases, nucleosides, and nucleotides are slightly different. The ultraviolet absorption peak of the nucleic acid is around 260 nm and can be used for the determination of nucleic acids. The purity of the nucleic acid can be judged based on the absorbance at 260 nm and 280 nm (A260).
Talk about protein, the composition of which is amino acid, the kind of which contains tyrosine, phenylalanine and tryptophan. The benzene ring of these aromatic amino acids contains conjugated double bonds, so that the protein has ultraviolet light absorption. nature. The absorption peak is at 280 nm, and its absorbance (ie, optical density value) is proportional to the protein content. Therefore, the ultraviolet absorption method is a light absorption method at 280 nm, and a protein solution containing a nucleic acid is preferably a absorption difference method of 280 and 260 nm. The dilute solution of the protein uses an absorption difference of 215 and 225 nm.
In addition, the light absorption value of the protein solution at 238 nm is proportional to the peptide bond content. The protein content can be determined by using a proportional relationship between the light absorption value of the protein solution and the protein concentration at a certain wavelength. The UV absorption method is simple, sensitive and fast, does not consume samples, and can be recycled after measurement.
There are also methods for determining proteins by producing a color by the action of proteins and some substances, and then measuring the absorbance in this colored light spectrum. The known concentration of the protein solution is diluted several times, and after reacting with the substance as a standard, the absorbance is measured to make a curve, and then the concentration of the unknown solution can be obtained by the absorbance. These methods include the Kjeldahl method, the Coomassie Brilliant Blue method (Bradford), the Folin-phenol reagent method (Lowry method), and the BCA method.
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